Hand hygiene is crucial for preventing the spread of infection worldwide. In many countries, the principles of the World Health Organization’s 7-step hand hygiene in hospitals have been adopted to prevent disease transmission in healthcare settings. In the past, hand hygiene was assessed by visually inspecting hands using fluorescent agents with trained evaluators, which could lead to human errors and require a significant amount of personnel. Therefore, the inventor devised a hand hygiene testing device to enhance the efficiency of hand cleanliness inspection among healthcare workers in hospitals, ensuring accuracy, precision, and speed.